Dracula’s mistress: removal of blood-red floral nectar results in secretion of more nectar
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Keywords

coloured nectar
Jaltomata
nectar removal
nectar replenishment
Solanaceae

How to Cite

Mione, T. and Diaz, I. (2020) “Dracula’s mistress: removal of blood-red floral nectar results in secretion of more nectar”, Plant Ecology and Evolution, 153(1), pp. 59-66. doi: 10.5091/plecevo.2020.1589.

Abstract

Background and aims – Flowers of Jaltomata quipuscoae (Solanaceae) secrete blood-red nectar that serves as an energy reward and possible attractant to pollinators. The purposes of this study were to determine whether simulated pollinator visits (manual removal of nectar) stimulates replenishment of nectar, and report the pattern of nectar presentation during the lifespan of the flower.
Methods – For the nectar replenishment experiments flowers were paired: each pair of flowers was selected to be on the same plant and at the same developmental stage. From all 62 flowers nectar was removed and discarded (not measured) at time zero. Then, over a period of eight hours, the nectar of one flower was measured four times, i.e., every two hours, while nectar of the paired control flower was measured only at the end of the eight-hour period. In the nectar dynamics experiment five sets of flowers received different treatments: flowers were unmanipulated for zero, one, two, three or four days and then nectar was removed once every day. The volume of nectar produced and concentration of sugar in the nectar were recorded at each extraction for both studies.
Key results – In the nectar replenishment study significantly higher nectar volume and consequently significantly higher total sugar content was present in the experimental nectar-extracted flowers. In the nectar dynamics study, nectar was produced starting on day one or two, continuously through the life of the open flowers until one or two days before the corolla senesced. Delay of nectar removal from different flower sets for zero, one, two, three or four days resulted in a linear increase in nectar volume and total nectar sugar production, and had little or no effect on the cumulative (life of the flower) nectar production. Floral longevity, seven to ten days, was not affected by a single removal of nectar each day.
Conclusions – The floral nectary of J. quipuscoae responded to nectar removal by secreting more nectar, and thus more total sugar (not a higher concentration of sugar) than was secreted by control flowers. In flowers from which nectar was not removed, nectar volume and thus total sugar secreted continued to accumulate linearly, suggesting that reabsorption of nectar either does not occur or is slow relative to the rate of secretion. The more we (or pollinators) take, the more the flowers make: the volume of nectar and sugar production increase if nectar is removed frequently but not if nectar is removed infrequently.

https://doi.org/10.5091/plecevo.2020.1589
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References

Bernardello L., Galetto L., Rodriguez I.G. (1994) Reproductive biology, variability of nectar features and pollination of Combretum fruticosum (Combretaceae) in Argentina. Botanical Journal of the Linnean Society 114(3): 293–308. https://doi.org/10.1111/j.1095-8339.1994.tb01938.x

Boose D.L. (1997) Sources of variation in floral nectar production rate in Epilobium canum (Onagraceae): implications for natural selection. Oecologia 110: 493–500. https://doi.org/10.1007/s004420050185

Carroll A.B., Pallardy S.G., Galen C. (2001) Drought stress, plant water status, and flora trait expression in fireweed, Epilobium angustifolium (Onagraceae). American Journal of Botany 88(3): 438–446. https://doi.org/10.2307/2657108

Castellanos M.C., Wilson P., Thomson J.D. (2002) Dynamic nectar replenishment in flowers of Penstemon (Scrophulariaceae). American Journal of Botany 89(1): 111–118. https://doi.org/10.3732/ajb.89.1.111

Castro S., Silveira P., Navarro L. (2008) Consequences of nectar robbing for the fitness of a threatened plant species. Plant Ecology 199: 201–208. https://doi.org/10.1007/s11258-008-9424-z

Corbet S. (2003) Nectar sugar content: estimating standing crop and secretion rate in the field. Apidologie 34: 1–10. https://doi.org/10.1051/apido:2002049

Cruden R.W., Hermann S.M., Peterson S. (1983) Patterns of nectar production and plant-pollinator coevolution. In: Bentley B., Elias T. (eds) The biology of nectaries: 80–125. New York, Columbia University Press.

Frohlich M.W. (1976) Appearance of vegetation in ultraviolet light: absorbing flowers, reflecting backgrounds. Science, New Series 194(4267): 839–841. https://doi.org/10.1126/science.194.4267.839

Galetto L., Bernardello L. (1992) Nectar secretion pattern and removal effects in six Argentinean Pitcairnioideae (Bromeliaceae). Botanica Acta 105(4): 292–299. https://doi.org/10.1111/j.1438-8677.1992.tb00301.x

Galetto L., Bernardello L. (1993) Nectar secretion pattern and removal effects in three species of Solanaceae. Canadian Journal of Botany 71(10): 1394–1398. https://doi.org/10.1139/b93-167

Galetto L., Bernardello G. (2005) Nectar. In: Dafni A., Kevan P., Husband B.C. (eds) Practical pollination biology: 261–313. Cambridge, Ontario, Canada, Enviroquest.

Gallagher M.K., Campbell D.R. (2017) Shifts in water availability mediate plant-pollinator interactions. New Phytologist 215(2): 792–802. https://doi.org/10.1111/nph.14602

GraphPad (2019) Prism, version 8.2.1. GraphPad Software, San Diego, California, USA. Available at https://www.graphpad.com/scientific-software/prism/ [accessed 23 Jan. 2020].

Hansen D.M., Olesen J.M., Mione T., Johnson S.D., Müller C.B. (2007) Coloured nectar: distribution, ecology, and evolution of an enigmatic floral trait. Biological Reviews 82(1): 83–111. https://doi.org/10.1111/j.1469-185X.2006.00005.x

Harder L.D., Barrett S.C.H. (1996) Pollen dispersal and mating patterns in animal-pollinated plants. In: Lloyd D.G., Barrett S.C.H. (eds) Floral biology: studies of floral evolution in animal-pollinated plants: 140–190. New York, Chapman and Hall.

Heinrich B., Raven P.H. (1972) Energetics and pollination ecology. Science 176(4035): 597–602. https://doi.org/10.1126/science.176.4035.597

Holmgren C.A., Betancourt J.L., Rylander K.A., Roque J., Tovar O., Zeballos H., Linares E., J. Quade (2001) Holocene vegetation history from fossil rodent middens near Arequipa, Peru. Quaternary Research 56(2): 242–251. https://doi.org/10.1006/qres.2001.2262

Kostyun J.L., Moyle L.C. (2017) Multiple strong postmating and intrinsic postzygotic reproductive barriers isolate florally diverse species of Jaltomata (Solanaceae). Evolution 71(6): 1556–1571. https://doi.org/10.1111/evo.13253

Leiva González S., Mione T., Yacher L. (2016) Especies de Jaltomata Schltdl. (Solanaceae) con néctar rojo, tres nuevas combinaciones nomenclaturales y un nuevo taxón del noreste de Perú. Arnaldoa 23(1): 21–98.

Luo E.Y., Ogilvie J.E., Thomson J.D. (2014) Stimulation of flower nectar replenishment by removal: A survey of eleven animal-pollinated plant species. Journal of Pollination Ecology 12(7): 52–62. https://doi.org/10.26786/1920-7603(2014)2

Mione T., Anderson G.J. (1996) Jaltomata: An introduction, and preliminary observations on the red/orange floral nectar. Solanaceae Newsletter, the New York Botanical Garden 4(2): 51–57.

Mione T., Anderson G.J. (2017) Genetics of floral traits of Jaltomata procumbens (Solanaceae). Brittonia 69: 1–10. https://doi.org/10.1007/s12228-016-9447-z

Mione T, Leiva González S. (1997) A new Peruvian species of Jaltomata (Solanaceae) with blood-red floral nectar. Rhodora 99(900): 283–286. https://www.jstor.org/stable/23313303

Mione T. (1999) Jaltomata II: new combinations for five South American species (Solanaceae). Brittonia 51: 31–33. https://doi.org/10.2307/2666553

Mione T., Leiva González S., Yacher L. (2000) Three new species of Jaltomata (Solanaceae) from Ancash, Peru. Novon 10(1): 53–59. https://doi.org/10.2307/3393185

Mione T., Mugaburu D., Connolly B. (2001) Rediscovery and floral biology of Jaltomata biflora (Solanaceae). Economic Botany 55(1): 167–168. https://www.jstor.org/stable/4256397

Mione T., Leiva González S., Yacher L. (2004) Jaltomata andersonii (Solanaceae): a new species of Peru. Rhodora 106: 118–123.

Mione T., Leiva González S., Yacher L. (2007) Five new species of Jaltomata (Solanaceae) from Cajamarca, Peru. Novon 17(1): 49–58. https://doi.org/10.3417/1055-3177(2007)17%5B49:FNSOJS%5D2.0.CO;2

Mione T., Leiva González S., Yacher L. (2015) Two new Peruvian species of Jaltomata (Solanaceae, Solaneae) with red floral nectar. Brittonia 67: 105–112. https://doi.org/10.1007/s12228-014-9360-2

Mione T., Leiva González S., Yacher L. (2016) The Jaltomata (Solanaceae) of Department Lima, Peru. Scholars Bulletin 2(8): 476–484.

Mione T., Leiva González S., Yacher L. (2018) Red floral nectar that absorbs ultraviolet light is produced by a new Peruvian species, Jaltomata weigendiana (Solanaceae). Phytologia 100(1): 12–18.

Mione T, Kostyun J., Leiva González S. (2019) Breeding system features and a novel method for locating floral nectar secretion in a South American nightshade (Jaltomata quipuscoae). Plant Biosystems. https://doi.org/10.1080/11263504.2019.1578277

Navarro L. (1999) Pollination ecology and effect of nectar removal in Macleania bullata (Ericaceae). Biotropica 31(4): 618–625. https://doi.org/10.1111/j.1744-7429.1999.tb00410.x

Nepi M., Cresti L., Guarnieri M., Pacini E. (2011) Dynamics of nectar production and nectar homeostasis in male flowers of Cucurbita pepo L. International Journal of Plant Sciences 172(2): 183–190. https://doi.org/10.1086/657648

Ordano M., Ornelas J.F. (2004) Generous-like flowers: nectar production in two epiphytic bromeliads and a meta-analysis of removal effects. Oecologia 140: 495–505. https://doi.org/10.1007/s00442-004-1597-0

Ordano M., Ornelas J.F. (2005) The cost of nectar replenishment in two epiphytic bromeliads. Journal of Tropical Ecology 21(5): 541–547. https://doi.org/10.1017/S026646740500266X

Ornelas J.F., Ordano M., Lara C. (2007) Nectar removal effects on seed production in Moussonia deppeana (Gesneriaceae), a hummingbird-pollinated shrub. Ecoscience 14(1): 117–123. https://doi.org/10.2980/1195-6860(2007)14%5B117:NREOSP%5D2.0.CO;2

Plourd K.C., Mione T. (2016) Pollination does not affect floral nectar production, and is required for fruit-set by a hummingbird-visited Andean plant species. Phytologia 98(4): 313–317.

Prys-Jones O.E., Corbet S.A. (1987) Bumblebees. Cambridge, Pelagic Publishing.

Pyke G.H. (1991) What does it cost a plant to produce floral nectar? Nature 350: 58–59. https://doi.org/10.1038/350058a0

Thorp R.W., Briggs D.L., Estes J.R., Erickson E.H. (1975) Nectar fluorescence under ultraviolet irradiation. Science, New Series 189(4201): 476–478. https://doi.org/10.1126/science.189.4201.476

Wu M., Kostyun J.L., Moyle L.C. (2019) Genome sequence of Jaltomata addresses rapid reproductive trait evolution and enhances comparative genomics in the hyper-diverse Solanaceae. Genome Biology and Evolution 11(2): 335–349. https://doi.org/10.1093/gbe/evy274

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